The latest edition offers greatly expanded coverage of directed mutagenesis and protein engineering, therapeutic agents, and genetic engineering of plants. Updated chapters reflect recent developments in biotechnology and the societal issues related to it, such as cloning, gene therapy, and patenting and releasing genetically engineered organisms. Over 480 figures, including 200 that are new in this edition, illustrate all key concepts. "Milestones" summarize important research papers in the history of biotechnology and their effects on the field. As in previous editions, the authors clearly explain all concepts and techniques to provide maximum understanding of the subject, avoiding confusing scientific jargon and excessive detail wherever possible. Each chapter concludes with a summary, references, and review questions. Ideally suited as a text for third- and fourth-year undergraduates as well as graduate students, this book is also an excellent reference for health professionals, scientists, engineers, or attorneys interested in biotechnology.

    Winner, Southwest Book Award, Border Regional Library Association, 2011What do you call a place where people are tortured and murdered and buried in the backyard of a nice, middle-class condo? Where police work for the drug cartels? Where the meanings of words such as "border" and "crime" and "justice" are emptying out into the streets and flowing down into the sewers? You call it Juárez or, better yet, Dreamland.Realizing that merely reporting the facts cannot capture the massive disintegration of society that is happening along the border, Charles Bowden and Alice Leora Briggs use nonfiction and sgraffito drawings to depict the surreality that is Ciudad Juárez, Mexico. Starting from an incident in which a Mexican informant for the U.S. Department of Homeland Security murdered a man while U.S. agents listened in by cell phone—and did nothing to intervene—Bowden forcefully and poetically describes the breakdown of all order in Juárez as the power of the drug industry outstrips the power of the state. Alice Leora Briggs's drawings—reminiscent of Northern Renaissance engraving and profoundly disquieting—intensify the reality of this place where atrocities happen daily and no one, neither citizens nor governments, openly acknowledges them.With the feel of a graphic novel, the look of an illuminated medieval manuscript, and the harshness of a police blotter, Dreamland captures the routine brutality, resilient courage, and rapacious daily commerce along the U.S.-Mexico border.

    It's bad for society, especially now. . . . Call me crotchety, but I can't help asking, whatever happened to the social contract?'The get-rich-quick exuberance of the late nineties may have temporarily blinded us to how dependent we are on one another. Subsequent events serve as reminders that the strength of our economy and the security of our society rest on the bonds that connect us. But what, specifically, are these bonds? What do we owe one another as members of the same society?With his characteristic humor, humanity, and candor, one of the nation's most distinguished public leaders and thinkers delivers a fresh vision of politics by returning to basic American values: workers should share in the success of their companies; those who work should not have to live in poverty; and everyone should have access to an education that will better their chances in life.An insider who knows how the economy and government really work, Reich combines realistic solutions with democratic ideals. Businesses do have civic responsibilities, and government must stem a widening income gap that threatens to stratify our nation. And everyone must get involved to help return us to a society that works for everyone.

    

     If you've been on social media lately, or turned on your TV, you may have noticed a lot of dumb ideas floating around."We know when history will repeat and when it won't.""We can tell the difference between evidence and coincidences.""The simplest explanation is usually true."Wrong, wrong, and dangerous!If we're not careful, loserthink would have us believe that every Trump supporter is a bigoted racist, addicts should be responsible for fixing the opioid epidemic, and that your relationship fell apart simply because you chewed with your mouth open.Even the smartest people can slip into loserthink's seductive grasp. This book will teach you how to spot and avoid it--and will give you scripts to respond when hollow arguments are being brandished against you, whether by well-intentioned friends, strangers on the internet, or political pundits. You'll also learn how to spot the underlying causes of loserthink, like the inability to get ego out of your decisions, thinking with words instead of reasons, failing to imagine alternative explanations, and making too much of coincidences.Your bubble of reality doesn't have to be a prison. This book will show you how to break free--and, what's more, to be among the most perceptive and respected thinkers in every conversation.

    

    He solidified his standing as the nation's foremost political forecaster with his near perfect prediction of the 2012 election. Silver is the founder and editor in chief of FiveThirtyEight.com. Drawing on his own groundbreaking work, Silver examines the world of prediction, investigating how we can distinguish a true signal from a universe of noisy data. Most predictions fail, often at great cost to society, because most of us have a poor understanding of probability and uncertainty. Both experts and laypeople mistake more confident predictions for more accurate ones. But overconfidence is often the reason for failure. If our appreciation of uncertainty improves, our predictions can get better too. This is the "prediction paradox": The more humility we have about our ability to make predictions, the more successful we can be in planning for the future.In keeping with his own aim to seek truth from data, Silver visits the most successful forecasters in a range of areas, from hurricanes to baseball, from the poker table to the stock market, from Capitol Hill to the NBA. He explains and evaluates how these forecasters think and what bonds they share. What lies behind their success? Are they good-or just lucky? What patterns have they unraveled? And are their forecasts really right? He explores unanticipated commonalities and exposes unexpected juxtapositions. And sometimes, it is not so much how good a prediction is in an absolute sense that matters but how good it is relative to the competition. In other cases, prediction is still a very rudimentary-and dangerous-science.Silver observes that the most accurate forecasters tend to have a superior command of probability, and they tend to be both humble and hardworking. They distinguish the predictable from the unpredictable, and they notice a thousand little details that lead them closer to the truth. Because of their appreciation of probability, they can distinguish the signal from the noise.

    With access to the Obama re-election machine, Michael Hastings reports the behind-the-scenes story of the campaign trail: from Obama's self-destructive performance at the first debate to the harrowing days of Hurricane Sandy, all culminating in his triumphant victory late in the evening on November 6th. Along the way, Hastings gives a first hand account of the excitement and madness traveling with the White House press corps, bringing to life a series of unforgettably strange moments from the trail. From one of the sharpest, funniest, and most controversial young American journalists writing today comes "Panic: 2012" - the definitive account of how President Obama almost blew it.

    His assailant ran down the street, jumped into an SUV, and vanished, hoping to join the vast majority of killers in American cities who are never arrested for their crimes. But as soon as the case was assigned to Detective John Skaggs, the odds shifted. Here is the kaleidoscopic story of the quintessential American murder--one young black man slaying another--and a determined crew of detectives whose creed was to pursue justice at all costs for its forgotten victims. Ghettoside is a fast-paced narrative of a devastating crime, an intimate portrait of detectives and a community bonded in tragedy, and a surprising new lens into the great subject of murder in America--why it happens and how the plague of killings might yet be stopped.

    Progressives claimed they knew how to solve homelessness, inequality, and crime. But in cities they control, progressives made those problems worse.Michael Shellenberger has lived in the San Francisco Bay Area for thirty years. During that time, he advocated for the decriminalization of drugs, affordable housing, and alternatives to jail and prison. But as homeless encampments spread, and overdose deaths skyrocketed, Shellenberger decided to take a closer look at the problem.What he discovered shocked him. The problems had grown worse not despite but because of progressive policies. San Francisco and other West Coast cities -- Los Angeles, Seattle, Portland -- had gone beyond merely tolerating homelessness, drug dealing, and crime to actively enabling them.San Fransicko reveals that the underlying problem isn't a lack of housing or money for social programs. The real problem is an ideology that designates some people, by identity or experience, as victims entitled to destructive behaviors. The result is an undermining of the values that make cities, and civilization itself, possible.

    Bannon had long sensed that deep within America's soul lurked an explosive tension. Cambridge Analytica had the data to prove it, and in 2016 Bannon had a presidential campaign to use as his proving ground.Christopher Wylie might have seemed an unlikely figure to be at the center of such an operation. Canadian and liberal in his politics, he was only twenty-four when he got a job with a London firm that worked with the U.K. Ministry of Defense and was charged putatively with helping to build a team of data scientists to create new tools to identify and combat radical extremism online. In short order, those same military tools were turned to political purposes, and Cambridge Analytica was born. Wylie's decision to become a whistleblower prompted the largest data crime investigation in history. His story is both exposé and dire warning about a sudden problem born of very new and powerful capabilities. It has not only exposed the profound vulnerabilities and profound carelessness in the enormous companies that drive the attention economy, it has also exposed the profound vulnerabilities of democracy itself. What happened in 2016 was just a trial run. Ruthless actors are coming for your data, and they want to control what you think.

    

    An American Iliad in the guise of contemporary political reportage, What It Takes penetrates the mystery at the heart of all presidential campaigns: How do presumably ordinary people acquire that mixture of ambition, stamina, and pure shamelessness that makes a true candidate? As he recounts the frenzied course of the 1988 presidential race -- and scours the psyches of contenders from George Bush and Robert Dole to Michael Dukakis and Gary Hart -- Pulitzer Prize-winning journalist Richard Ben Cramer comes up with the answers, in a book that is vast, exhaustively researched, exhilarating, and sometimes appalling in its revelations.

    Following on the success of his best-selling The Future of Freedom, Zakaria describes with equal prescience a world in which the United States will no longer dominate the global economy, orchestrate geopolitics, or overwhelm cultures. He sees the "rise of the rest"—the growth of countries like China, India, Brazil, Russia, and many others—as the great story of our time, and one that will reshape the world. The tallest buildings, biggest dams, largest-selling movies, and most advanced cell phones are all being built outside the United States. This economic growth is producing political confidence, national pride, and potentially international problems. How should the United States understand and thrive in this rapidly changing international climate? What does it mean to live in a truly global era? Zakaria answers these questions with his customary lucidity, insight, and imagination.

    The highs, the lows, historic match days and foul play. It’s a celebration of a beloved player, a history maker, and his tragic ending at the final whistle. It’s a story about science and discovery, about the people on the front line of life-changing research. But above all it’s a story about family and about consequence. All of this is wrapped up in three little letters: CTE. An investigative documentary from multi-award-winning producer Hana Walker-Brown [Audio Production Awards Best Documentary (Winner, 2017, 2016), Prix Europa (Nominee, 2018, 2017, 2015), Åke Blomström Prize (Winner, 2015) Hearsay International Audio Arts Award (Nominee, 2014) and Charles Parker Prize Gold Award (Winner, 2013)], The Beautiful Brain looks at the devastating effects of chronic traumatic encephalopathy (CTE) - a degenerative brain disease found in people who’ve suffered severe or repeated blows to the head. Beginning with the story of FA Cup winner Jeff Astle, the audiobook expands outwards looking at the widows, daughters and families who are left fighting for answers following the death of their loved ones. As the documentary progresses, Hana’s investigation leads her from Britain’s football pitches to support groups for victims of domestic violence and beyond, sparking a vital discussion about accountability and the urgent need for further scientific studies into CTE.Running time: 3 hrs and 42 mins©2019 Audible, Ltd (P)2019 Audible, Ltd