With the assistance of a co-author, this popular student textbook has now been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.

    Life remains the only way to make life. Are we still missing a vital ingredient in its creation?      Like Richard Dawkins' The Selfish Gene, which provided a new perspective on how evolution works, Life on the Edge alters our understanding of our world's fundamental dynamics. Bringing together first-hand experience at the cutting edge of science with unparalleled gifts of explanation, Jim Al-Khalili and Johnjoe Macfadden reveal that missing ingredient to be quantum mechanics; the phenomena that lie at the heart of this most mysterious of sciences. Drawing on recent ground-breaking experiments around the world, each chapter in Life on the Edge engages by illustrating one of life's puzzles: How do migrating birds know where to go? How do we really smell the scent of a rose? How do our genes copy themselves with such precision? Life on the Edge accessibly reveals how quantum mechanics can answer these probing questions of the universe. Guiding the reader through the rapidly unfolding discoveries of the last few years, Al-Khalili and McFadden communicate the excitement of the explosive new field of quantum biology and its potentially revolutionary applications, while offering insights into the biggest puzzle of all: what is life? As they brilliantly demonstrate in these groundbreaking pages, life exists on the quantum edge.

    Often the age-old customs, whose relevance is lost to modern times, are dismissed as meaningless superstitions. The truth, however, is that these practices reveal the philosophical and scientific approach to life that has characterized Hindu thought since ancient times; it is important to revive their original meanings today. This handy book tells the fascinating stories and explains the science behind the Hindu rites and rituals that we sometimes follow blindly. It is essential reading for anyone interested in India's cultural tradition.

    That unfortunate situation began to change when Einstein published several of the papers and speeches contained in this book which explain the central core of the theory in clear and often beautiful language accessible to any interested reader.In addition to lucid explications of both the Special and General theories, Einstein holds forth on the principles of research, the nature of scientific truth, and the method of theoretical physics. He also offers acute analyses and appreciation of the work of such giants as Johannes Kepler, Isaac Newton, Clerk Maxwell, and Niels Bohr.

    have collected more than a thousand passages from the works of Mr. Ikeda and categorized them by subject for easy reference ... find relevant quotes on topics of importance to the Buddhist practitioner and the concerned citizen of the world"--P. ix.

    In Zero to One, legendary entrepreneur and investor Peter Thiel shows how we can find singular ways to create those new things. Thiel begins with the contrarian premise that we live in an age of technological stagnation, even if we’re too distracted by shiny mobile devices to notice. Information technology has improved rapidly, but there is no reason why progress should be limited to computers or Silicon Valley. Progress can be achieved in any industry or area of business. It comes from the most important skill that every leader must master: learning to think for yourself.Doing what someone else already knows how to do takes the world from 1 to n, adding more of something familiar. But when you do something new, you go from 0 to 1. The next Bill Gates will not build an operating system. The next Larry Page or Sergey Brin won’t make a search engine. Tomorrow’s champions will not win by competing ruthlessly in today’s marketplace. They will escape competition altogether, because their businesses will be unique. Zero to One presents at once an optimistic view of the future of progress in America and a new way of thinking about innovation: it starts by learning to ask the questions that lead you to find value in unexpected places.

    Hailed as "unsinkable, " the "Kursk" was on maneuvers when mysterious explosions rocked the sub, causing it to sink to the bottom of the sea with its 118-man crew. This in-depth look at the disaster reveals previously unreleased information from family members of the deceased as well as from government officials.

    You tell your friends about them later, not sure exactly what it was about them that impressed you so much.I make an effort to surround myself with this kind of person because I’ve gained so much from them in the past. Just by living their lives they make me work harder and push myself to achieve more, to be happier, to live a fuller, richer life.Over the years I’ve picked up a lot of useful pieces of advice from these people, while at the same time observing and trying to figure out what makes them tick. I’ve worked to apply many of these same lessons to my own life and have seen very positive changes as a result.I wrote this book to share some of these tidbits of knowledge with everyone because I firmly believe that if the world has more inspiring, ambitious and remarkable people we’ll all be a lot better off.

    Especially in the West, we have created a frenzied lifestyle in which the twenty-­four hours of each day are carved up, dissected, and reduced down to ten minute units of efficiency. We take our iPhones and laptops with us on vacation. We check email at restaurants or our brokerage accounts while walking in the park. When the school day ends, our children are overloaded with “extras.” Our university curricula are so crammed our young people don’t have time to reflect on the material they are supposed to be learning. Yet in the face of our time-driven existence, a great deal of evidence suggests there is great value in “wasting time,” of letting the mind lie fallow for some periods, of letting minutes and even hours go by without scheduled activities or intended tasks. Gustav Mahler routinely took three or four-­hour walks after lunch, stopping to jot down ideas in his notebook. Carl Jung did his most creative thinking and writing when he visited his country house. In his 1949 autobiography, Albert Einstein described how his thinking involved letting his mind roam over many possibilities and making connections between concepts that were previously unconnected. With In Praise of Wasting Time, Professor Alan Lightman documents the rush and heave of the modern world, suggests the technological and cultural origins of our time-­driven lives, and examines the many values of “wasting time”—for replenishing the mind, for creative thought, and for finding and solidifying the inner self. Break free from the idea that we must not waste a single second, and discover how sometimes the best thing to do is to do nothing at all.

    This is a fresh edition containing a wealth of new material that will enhance its already considerable reputation.

    Gelb identified seven aspects of Da Vinci's genius that contemporary readers can emulate and apply in their own lives. Now, in WORK LIKE DA VINCI, Gelb adapts these principles to the specific demands of the workplace, sharing the innovative solutions to contemporary corporate and career challenges that have kept him in constant demand as a top-tier speaker and consultant to Fortune 500 clients. In Gelb's expert perspective, Da Vinci's genius can be distilled into seven principles for the business listener: Ask the right questions (Curiosit�) Put your answers to work (Dimostrazione) Develop your business senses (Sensazione) Turn uncertainty into opportunity (Sfumato) Strike a profitable balance (Arte/Scienza) Integrate for success (Corporalit�) Make the break-through connection (Connessione) These principles will help you tackle such timeless business challenges as: leadership; innovation; teamwork; strategic planning; decision-making; managing change and uncertainty; giving powerful presentations; giving and receiving feedback; and more.

    Scientific Shaman. Big Brother Au-Go-Go. He sits comfortably at the apex of the pyramid of worldly knowledge, twiddling his thumbs. His word, according to followers, is The Word, and that word is Slack. Beyond science, reason — and orgasm — find in Bobliography instant instructions for those who follow no master. The third installment of the holy SubGenius books, Bobliography is an uproarious send-up of all things cult. In addition to providing a guide for Eternal Salvation (or triple your money back), Bobliography is the encapsulated history of the SubGenius "movement" — from its beginnings in the 1980s to the growing Internet empire it has lately become — and also the essential, comprehensive collection of SubGenius lore. A Whole Earth catalog for the Deeply Weird, a Farmer's Almanac for the Truly Strange — Bobliography is the revelation of the millennium.

    Real rationality, of the sort studied by psychologists, social scientists, and mathematicians. The kind of rationality where you make good decisions, even when it's hard; where you reason well, even in the face of massive uncertainty; where you recognize and make full use of your fuzzy intuitions and emotions, rather than trying to discard them. In "Rationality: From AI to Zombies," Eliezer Yudkowsky explains the science underlying human irrationality with a mix of fables, argumentative essays, and personal vignettes. These eye-opening accounts of how the mind works (and how, all too often, it doesn't!) are then put to the test through some genuinely difficult puzzles: computer scientists' debates about the future of artificial intelligence (AI), physicists' debates about the relationship between the quantum and classical worlds, philosophers' debates about the metaphysics of zombies and the nature of morality, and many more. In the process, "Rationality: From AI to Zombies" delves into the human significance of correct reasoning more deeply than you'll find in any conventional textbook on cognitive science or philosophy of mind. A decision theorist and researcher at the Machine Intelligence Research Institute, Yudkowsky published earlier drafts of his writings to the websites Overcoming Bias and Less Wrong. "Rationality: From AI to Zombies" compiles six volumes of Yudkowsky's essays into a single electronic tome. Collectively, these sequences of linked essays serve as a rich and lively introduction to the science—and the art—of human rationality.

    But the cliché is true: happiness truly is in the journey, not the destination.Psychologist Cassandra Dunn believes that happiness is available to all of us – and not just in some picture-perfect ideal life. Cass has helped thousands of people get from Crappy to Happy with her hit podcast. In this book Cass expands on those conversations and provides even more information and practical tools, helping you learn to let go, to find your people, to determine your direction and more.Your journey to living your best life begins right here and now.

    Waiting for Cawdor are two of his oldest enemies, ready to overtake the barony and the Cawdor name.Unable to help an ailing Krysty Wroth, Cawdor must face this challenge to the future of the East Coast baronies on his own.