With the assistance of a co-author, this popular student textbook has now been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.

    Google Hacking teaches people how to get the most out of this revolutionary search engine. Not only will this book teach readers how Google works, but it will also empower them with the necessary skills to make their everyday searches easier, more efficient, and more productive. Google Hacking also demonstrates how Google can be used for negative means. It's immense searching power, means that everyone, including cyber criminals, can feasibly access confidential data, such as company presentations, budgets, blueprints, even credit card numbers, with just the click of a mouse. Using numerous examples, case studies, and screenshots, this book explains the art of ethical Google Hacking -- it not only teaches readers how Google works, but it provides them with the knowledge they need to protect their data and systems from getting Google Hacked. This is the only book you need to maximize (and protect yourself) from Google searches!

    Cameron Webb’s brief but biting assault on the wide spectrum of religiosity that dominates 21st century America, from the hateful and anti-intellectual dogma of the Christian Right to the whitewashed progressivism of religious moderates. It is also a fascinating and humbling journey into the heart of the universe's most mind-numbing wonders.Drawing on recent insights from cosmology and evolution, Despicable Meme paints a vivid portrait of a cosmos unlike anything ever imagined by the provincial, human-centered faiths of the past – a universe of countless worlds spread across unfathomable distances and times, and where, on at least one of those worlds, the slow march of time would combine with the purposeless mechanisms of chemistry and physics to create a being capable of believing that he alone is the reason for it all.With piercing intelligence and candor, Despicable Meme exposes the folly of that conceit and dispenses with the widespread but utterly improbable notion of a personal creator. But it saves its harshest criticism for the vapid accommodationism of religious liberals, those who unknowingly or uncaringly give cover to the misogynistic, racist, homophobic paranoia of the fanatics by refusing to condemn, or quietly tolerating, the outlandish and immoral doctrines that lie festering at the center of their own “moderate” faiths.Despicable Meme is not only a blistering condemnation of radical fundamentalism, it is an impassioned appeal to the rest of us to once and for all abandon the superstitions of the religion we were raised in and embrace the beauty of an endlessly wondrous, but godless, universe.Show less

    Why did it take so long for him to win the Nobel Prize? What kind of a father was Einstein to his boys? How did his marriages affect his work? What motivated him? And most importantly; what unlocked his mind to grapple with the most profound ideas of all time? Inside you will read about... ✓ Einstein

    This is Nick's story of his world record-breaking adventure and the extraordinary people who joined him along the way. On January 6th 2018, Nick Butter tied his laces and stepped out on to an icy pavement in Toronto, where he began to take the first steps of an epic journey that would see him run 196 marathons in every one of the world's 196 countries. Spending almost two years on the road and relying on the kindness of strangers to keep him moving, Nick's odyssey allowed him to travel slowly, on foot, immersing himself in the diverse cultures and customs of his host nations. Running through capital cities and deserts, around islands and through spectacular landscapes, Nick dodges bullets in Guinea-Bissau, crosses battlefields in Syria, survives a wild dog attack in Tunisia and runs around an erupting volcano in Guatemala. Along the way, he is often joined by local supporters and fellow runners, curious children and bemused passers-by. Telling their stories alongside his own, Nick captures the unique spirit of each place he visits and forges a new relationship with the world around him. Running the World captures Nick's journey as he sets three world records and covers over five thousand miles. As he recounts his adventures, he shares his unique perspective on our glorious planet, celebrates the diversity of human experience, and reflects on the overwhelming power of running.

    You’ll find it fun to read too - written in simple, everyday language.And, if you’re like most people, you’ll find that learning “why” the law of attraction works will pour rocket fuel into your belief in it. And attaining such a level of belief will allow you to unleash the law of attraction more powerfully than you’ve done previously. Why Quantum Physicists Create More Abundance removes your barriers of doubt and resistance concerning the law of attraction. It can be a very powerful tool for you, helping you soar past previous frustrations and manifesting a life much more closely aligned with your dreams and desires.

    Everything around us, including matter, is energy. A deep look into the mysteries of the subatomic world – the particles that make up the atom – provides answers to basic questions about how the universe works. To solve the future of mankind’s energy needs we need to understand the basic building blocks of the universe, including the atom and its parts. By exploring the subatomic world we’ll find more answers to our questions about time, forces like gravity and the matter that surrounds us. More importantly, we’ll find new ways to tap into the energy that exists around us to power our growing needs. In a new branch of particle physics, where tiny particles are thought of as energy waves, we find new answers that may help us in our quest to find alternative energy sources.

    Assuming the reader has little prior knowledge of the subject its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented two-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology: Extended chapter on agriculture including new material on glyphosate resistant plantsNew section on the uses of gene cloning and PCR in archaeologyCoverage of ethical concerns relating to pharming, gene therapy and GM crops Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. View the Gene Cloning and DNA Analysis webpage at www.blackwellpublishing.com/genecloning

    Welcome to laughing until it hurts while covered in bodily fluids. Welcome to simple math at very high stakes. Welcome to an incredibly inappropriate sense of humor. Welcome to serving people on the most stressful days of their lives. Welcome to putting your hands in places you never imagined they'd be. Welcome to your front row seat to the ballad of life and death. That's not the welcome that this nurse was looking for, but that's the one he got. Irreverent and audacious, this brutally honest memoir covers what it’s like to come of age in an American Hospital. Welcome to a rollicking peak behind the curtain to what medical providers, and the health care system, are truly like.

    The narcissist provides the direct and no-nonsense explanations and answers to the questions which matter most to you. The narcissist manages to keep a hook in you by leaving you with unanswered questions. These questions prevent you from gaining understanding, make you susceptible to the pull of the narcissist in the future and cause you untold anguish and anxiety. Not any more. A range of incisive questions covering the narcissistic spectrum of behaviours have been posed by those who have been on the receiving end of narcissistic behaviour. Real questions posed by those who know exactly what it is like to be held in the grasp of the narcissist. Real answers provided by the narcissist himself which will provide understanding, enlightenment and freedom.

    But Max Leonard, himself an accomplished amateur cyclist, does not forget the pain, the glory, the sweat, and the tears that go into these grueling climbs.  After all, cycling up a mountain is hard.  So hard that, to many, it can seem absurd. But for others, climbing a mountain gracefully (and beating your competitors up the slope) represents the pinnacle of cycling achievement. It is where legends are forged.Many books tell you where the mountains are, or how long and how high. None of them ask why. Why are mountain ranges professional cycling’s Coliseum? Why do amateurs also make pilgrimages to these high, remote roads? Why are the roads even there in the first place to lure us on to these obsession inducing climbs?   Just why are mountains so enthralling? “This is real cycling, where the glory is and where dreams come true,” according to Bradley Wiggins. Mountains are where cycling's greatest heroes have made their names. Every amateur rider wishes they could climb better, too.  Are all these people addicted to the pain? To the achievement? Or to the allure of the peaks? Some spend their weekends and holidays cycling up mountains from start to finish. But how does a rider push themselves beyond their limits to get up a 10% gradient on pedal power alone? What is happening when they do?A Higher Calling explores the central place of mountains in the folklore of road cycling. Blending adventure and travel writing with the rich narrative of racing, Max Leonard takes the reader from the battles that created the Alpine roads to the shepherds tending their flocks on the peaks, and to a Grand Tour climax on the “highest road in Europe.” And he tells stories of courage and sacrifice, war and love, obsession and even elephants, along the way.

    Because of this balance, "Microbiology" is appropriate for microbiology majors and mixed majors courses. The new authors have focused on readability, artwork, and the integration of several key themes (including evolution, ecology and diversity) throughout the text, making an already superior text even better.

    

    Scott guides readers through the latest advances in stem cell research in clear, accessible language, telling the stories of the researchers who are exploring the potential of stem cells to cure cancer, grow new organs, and repair the immune system. He also leads readers through a discussion of the question at the heart of the explosive ethical debate: How, as a society, do we balance our responsibilities to the unborn and the sick? Stem Cell Now is essential reading for anyone who wants to build an informed opinion on stem cell research.

    Whether you are a patient struggling with miscarriages or a medical provider caring for patients with recurrent pregnancy loss, you will learn something from this resource. Dr. Shahine explains not only a typical Western medicine approach to evaluation and treatment for miscarriage but also includes Eastern approaches to care, lifestyle factors that will decrease your risk of miscarriage, and the emotional impact of recurrent pregnancy loss. You will finish this book feeling more empowered to be an advocate for your care and more hopeful than ever to continue towards your family goals. “I have one word to describe this fabulous book: FINALLY. Women with recurrent pregnancy loss have been needing this book for years.” – Dr. Alice Domar, associate professor of obstetrics, gynecology, and reproductive biology at Harvard Medical School and author of Conquering Infertility and Finding Calm for the Expectant Mom