Assuming the reader has little prior knowledge of the subject its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented two-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology: Extended chapter on agriculture including new material on glyphosate resistant plantsNew section on the uses of gene cloning and PCR in archaeologyCoverage of ethical concerns relating to pharming, gene therapy and GM crops Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. View the Gene Cloning and DNA Analysis webpage at www.blackwellpublishing.com/genecloning

    It is written by internationally recognized Harvard Medical School faculty and select medical students, and is the best text to bridge basic physiology with clinical care of patients.This edition provides updated coverage of pathogenesis of atherosclerosis, pathophysiology of acute coronary syndromes, mechanisms of heart failure, molecular mechanisms of dysrhythmias, the genomic basis of cardiomyopathies and congenital heart disease, and pharmacology. Numerous new illustrations are included.A companion Website on thePoint will include animations and audio heart sounds.

    It features new revelations about high-profile, unsolved hits and the intricate relationships between known criminals and police officers at all levels. It delves into the current power struggle between opposing factions in Cape Town's security industry and the suspected involvement of state operatives in the bloody standoff.Wiener has gained exclusive access to and on-the-record interviews with key underworld characters and police generals accused of colluding with criminals. These have helped her track the parallel narrative of the capture of law-enforcement agencies and unravel how players with inexplicable political backing have been able to pillage secret slush funds and abuse organs of state for their own benefit.Against this backdrop, prominent underworld figures - Radovan Krejcir key among them - have been able to thrive, setting up elaborate networks with the assistance of police. While crime is flourishing, the top echelons of the police and prosecution have been at war with themselves.The proximity of politics, law enforcement and organised crime over the past decade is frighteningly intertwined. The story of the rise and reign of the Ministry of Crime winds its way from the depths of the underworld, via multiple mysterious unsolved murders, to senior politicians and the very top ranks of the country's police force.

    Many of the difficulties however, are not in the classroom... In The (Un)official Teacher's Manual, Omar Akbar offers direct, humorous and accessible advice on how to deal with the daily issues faced by a teacher- none of which involve teaching! Includes guidance on: lesson observations, emails, promotions, avoiding meaningless extra work, meetings, parents, maintaining a work-life balance, dealing with workplace bullying, and much more. While Omar pulls no punches on the reality of working in a school, a positive streak is maintained throughout. A must read for any teacher or potential teacher. - - - - - - - - - - - - - - Contents: Introduction: Why This Book Was Written 1. How to Get the Most from Observations, Learning Walks, and Book Scrutinies 2. The Don’ts of the School Email System 3. How to Get Promoted and Other Things to Consider 4. How and When to Say No and Yes 5. The Dos and Don’ts of Meetings 6. How to Get Parents on Your Side 7. Guidance for Trainee Teachers 8. Ensuring a Life-Work Balance 9. Bullying: the Problem and the Solution 10. How to Get the Teaching Job You Want 11. Maintaining Good Relationships 12. Why It’s All Worth It

    In 1750, the anatomist John Hunter described it as “a humiliating spectacle of the futility of science”; yet, over the next 150 years the feared, practical men of medicine benefited from a revolution in scientific progress and the increased availability of instructional textbooks. Anesthesia and antisepsis were introduced. Newly established medical schools improved surgeons’ understanding of the human body. For the first time, surgical techniques were refined, illustrated in color, and disseminated on the printed page.Crucial Interventions follows this evolution, drawing from magnificent examples of rare surgical textbooks from the mid-nineteenth century. Graphic and sometimes unnerving yet beautifully rendered, these fascinating illustrations, acquired from the Wellcome Collection’s extensive archives, include step-by-step surgical techniques paired with depictions of medical instruments and depictions of operations in progress.Arranged for the layman (from head to toe) Crucial Interventions is a captivating look at the early history of one of the world’s most mysterious and macabre professions.

    Now including a new introduction explaining the impact of DSM-5 on the diagnosis and approach to AS, it brings together a wealth of information on all aspects of the syndrome for children through to adults.Drawing on case studies and personal accounts from Attwood's extensive clinical experience, and from his correspondence with individuals with AS, this book is both authoritative and extremely accessible. Chapters examine:* causes and indications of the syndrome* the diagnosis and its effect on the individual* theory of mind * the perception of emotions in self and others* social interaction, including friendships* long-term relationships* teasing, bullying and mental health issues* the effect of AS on language and cognitive abilities, sensory sensitivity, movement and co-ordination skills* career development.There is also an invaluable frequently asked questions chapter and a section listing useful resources for anyone wishing to find further information on a particular aspect of AS, as well as literature and educational tools.Essential reading for families and individuals affected by AS as well as teachers, professionals and employers coming in contact with people with AS, this book should be on the bookshelf of anyone who needs to know or is interested in this complex condition.'I usually say to the child, "Congratulations, you have Asperger's syndrome", and explain that this means he or she is not mad, bad or defective, but has a different way of thinking.' - from The Complete Guide to Asperger's Syndrome.

    The text is aimed at second- and third-year one-semester ethics courses offered in medical schools, health sciences departments, and nursing programs. By taking an applied approach rather than a theoretical approach, this text serves the needs of medical and nursing students, residents, and practicing physicians by sorting through questions of moral principles relevant to the diverse and growing number of healthcare professionals. The many topics covered include truth telling, refusal of treatment, assisted suicide, managing error, and reproductive choice.

    Economics, finance, business & management

    The Old Testament is filled with stories: Noah and the ark, Daniel in the lions' den, Ruth and Boaz—stories of great men and women of God.What does God want us to learn from these stories? Is there more to them than meets the eye? Richard L. Pratt says yes, and he carefully outlines a method for grasping the wisdom of these ageless narratives. Beginning with the role of the Holy Spirit, he sets forth a three-step process of biblical interpretation:–Preparation–Investigation–ApplicationPratt is sensitive to the needs of both the beginner and the theological student. He insightfully guides us through considerations of attitudes and orientation, language and history, writers and characters, scenes and events, ancient and modern cultures, and more.

    Following the huge success of Now Then Lad and You're Coming With Me Lad, Yorkshire bobby, Mike Pannett, returns with new crime fighting stories.

    She is falling in love with the ideal man while advancing in her professional career. Then her neat and ordered life crashes. She receives a strange email from her deceased grandmother. Coupled with this, her apartment is burgled and her cookbooks are stolen, the ones she was instructed to preciously guard. After hiring a quirky private detective she goes on a quest that leads to an impossible truth. This exciting action thriller is interlaced with memories of tastes, smells and laughter in the kitchen, and how a grandmother lovingly prepared a young girl to face enormous challenges. Not only did Kate learn the craft of cooking, but also values from old-world traditions. These savoury breaks in the story provide unusual twists not typically found in bestseller action-thriller novels. Those lessons passed from one generation to the next give Kate the strength to face powerful forces. While solving mysteries she is led on a journey of self-discovery, having to ask hard questions. What is this disconcerting game that her grandmother is playing and for what purpose? Is her past just an illusion? And, why are these malevolent people intent on extracting some unknown truth from her? There are unique and unexpected rewards with this book. Recipes for dishes in the novel are found on the author’s website, thereby prolonging the pleasant memories of the story. Kate is a gourmet cook and you can recreate her delectable delights, whether for a special candlelight dinner for two or for an entire family. ‘The Cookbook’ carries themes of romance and love, and of faith and hope as it explores the limits of how much trust one can give to others. When everyone is against you, how can you stand up with toughness and tenacity against the world? One reviewer wrote, “This book is like a superb meal. It’s a wonderful mix of the pleasures of cooking and the enjoyment of an action read. One can only say, ‘Bon Appetit!’” If you love an adrenaline driven escape found in a fun action story, this is it.

    Nearly a century ago it was the site of a gruesome murder which, to this day, resonates within its decaying walls.COMPASSION. REDEMPTION. IF SHE CAN FIND ONE, SHE CAN DELIVER THE OTHER.Tilly Bright is a girl with an extraordinary gift, one which she never wanted and which she has spent a lifetime trying to suppress. At twelve years old Tilly vowed she would never again set foot in her grandmother’s farmhouse. Never again would she allow her sensitive mind to be attacked and manipulated by the vengeful, hate-filled spirit that inhabits it. He is called The Shadow. Nothing remains of his humanity except for the rage that consumes him over his long-ago death.Upon her grandmother’s passing, however, Tilly has no choice—she must go back. Halloran Farm is now hers, as is a significant inheritance which is meant to help her restore the crumbling Victorian to its former beauty. It is what Gram wanted. And Tilly is no longer a frightened child unable to cope with a gift she does not understand. To fulfill Gram’s wishes, she will have to find within herself the strength to confront The Shadow. When she does, she will have to reconsider everything she thought she knew about her gift, about the dead, and about The Shadow himself.Even the most hateful spirits deserve compassion. For through it, they may find their way to redemption.

    

    But in the aftermath, Avi is forced to flee to America. His darling wife Sara and the rest of his family soon follow – all except his brother Lieb, who stubbornly refuses to abandon his home. In ensuing years, while Avi lives the American immigrant’s dream, Lieb lives Russia’s nightmare: World War I, the Communist revolution, civil war, typhus, and famine. Still Lieb rejects Avi’s pleas to leave Russia. Then on the eve of World War II, Stalin’s pathological purges finally ensnare Lieb’s family. At last he realizes he must escape the Communist nightmare, but now all avenues are blocked, and Hitler’s armies are gathering. He turns to Avi, his brother in America, who frantically tries to rescue Lieb and his family with little more to work with than his own wit. Stretching from pre-Revolution Russia to post-Holocaust America, A Fine September Morning blends historical facts and fictional characters into a compelling epic family saga.

    With swashbuckling wit and fierce honesty, she dishes on herself and her colleagues as they try to prod reluctant governments to fund HIV prevention for the people who need it most—drug injectors, gay men, sex workers, and johns.Pisani chats with flamboyant Indonesian transsexuals about their boob jobs and watches Chinese streetwalkers turn away clients because their SUVs aren't nice enough. With verve and clarity, she shows the general reader how her profession really works; how easy it is to draw wrong conclusions from "objective" data; and, shockingly, how much money is spent so very badly. "Exhibit A": the 45 billion taxpayer dollars the Bush administration is committing to international AIDS programs.