Assuming the reader has little prior knowledge of the subject its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented two-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology: Extended chapter on agriculture including new material on glyphosate resistant plantsNew section on the uses of gene cloning and PCR in archaeologyCoverage of ethical concerns relating to pharming, gene therapy and GM crops Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. View the Gene Cloning and DNA Analysis webpage at www.blackwellpublishing.com/genecloning

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    These tools, which are the foundation of his Own Anxiety method lead, to a redefined relationship with anxiety, taking it from negative and debilitative to positive and facilitative. Rather than trying to fight it, Brass encourages readers to consider anxiety a gift. Personal, personable, and highly motivating, the book offers practical lessons to overcome the effects of anxiety by owning it in three key areas of body, mind and soul.Combining medical research from the West and holistic philosophies from the East, the tips offer daily doses of inspiration. From guidance on how to schedule smarter to reduce stress, how to say no more often, and how to rein in the use of social media to advice about spreading love and playing more often, the book makes owning anxiety both accessible and fun. Warm, enthusiastic, and at times irreverent, Brass offers stories from his own life and shows from personal experience how to conquer anxiety. He is a coach of superlative positivity, encouraging readers to find the resources within to master anxiety toward a more fulfilling and healthier lifestyle.

    Rejecting the idea that we should live in fear, suffer silently, or medicate ourselves unnecessarily through this hormonal shift, Mathews set out to get answers and advice from the medical establishment, alternative therapists, and her many friends in the midst of "the change." When she launched the Megs Menopause website, it quickly became the trending online destination for pre- and menopausal women all over the world.Now, in The New Hot, Mathews offers the results of all her research and discussions: the latest information about hormone treatments (hormone replacement therapy and bioidentical hormone therapy), her best tips and techniques for coping with menopausal symptoms (there are officially thirty-four possible symptoms; Mathews has dealt with thirty-two!), and dishy, girlfriend-to-girlfriend advice about what to really expect when you're aging. Entertaining, stylish, and informative, The New Hot will be the resource women everywhere are talking about, learning from, and recommending to one another.

    It is the root of our existence, supporting our feet, our farms, our cities. This fascinating yet disquieting book finds, however, that we are running out of dirt, and it's no laughing matter. An engaging natural and cultural history of soil that sweeps from ancient civilizations to modern times, Dirt: The Erosion of Civilizations explores the compelling idea that we are—and have long been—using up Earth's soil. Once bare of protective vegetation and exposed to wind and rain, cultivated soils erode bit by bit, slowly enough to be ignored in a single lifetime but fast enough over centuries to limit the lifespan of civilizations. A rich mix of history, archaeology and geology, Dirt traces the role of soil use and abuse in the history of Mesopotamia, Ancient Greece, the Roman Empire, China, European colonialism, Central America, and the American push westward. We see how soil has shaped us and we have shaped soil—as society after society has risen, prospered, and plowed through a natural endowment of fertile dirt. David R. Montgomery sees in the recent rise of organic and no-till farming the hope for a new agricultural revolution that might help us avoid the fate of previous civilizations.

    News of both the Ice Man's discovery and his age, which was put at over five thousand years, fascinated scientists and newspapers throughout the world. But what made Sykes's story particularly revelatory was his successful identification of a genetic descendant of the Ice Man, a woman living in Great Britain today. How was Sykes able to locate a living relative of a man who died thousands of years ago?In The Seven Daughters of Eve, he gives us a firsthand account of his research into a remarkable gene, which passes undiluted from generation to generation through the maternal line. After plotting thousands of DNA sequences from all over the world, Sykes found that they clustered around a handful of distinct groups. Among Europeans and North American Caucasians, there are, in fact, only seven. This conclusion was staggering: almost everyone of native European descent, wherever they may live throughout the world, can trace their ancestry back to one of seven women, the Seven Daughters of Eve.Naming them Ursula, Xenia, Helena, Velda, Tara, Katrine, and Jasmine, Sykes has created portraits of their disparate worlds by mapping the migratory patterns followed by millions of their ancestors. In reading the stories of these seven women, we learn exactly how our origins can be traced, how and where our ancient genetic ancestors lived, and how we are each living proof of the almost indestructible strands of DNA, which have survived over so many thousands of years. Indeed, The Seven Daughters of Eve is filled with dramatic stories: from Sykes's identification, using DNA samples from two living relatives, of the remains of Tsar Nicholas and Tsaress Alexandra, to the Caribbean woman whose family had been sold into slavery centuries before and whose ancestry Sykes was able to trace back to the Eastern coast of central Africa. Ultimately, Sykes's investigation reveals that, as a race, what humans have in common is more deeply embedded than what separates us.

    What followed was a family journey of epic proportions – a journey of physical challenge, emotional endurance, teamwork, perseverance, and tremendous learning opportunities. It was a discovery of self, of priorities, of accepting hardships, of appreciating blessings, and of contrasting a comfortable past life with the extreme hardship and poverty of those they met.

    On March 27, 1964, at 5:36 p.m., the biggest earthquake ever recorded in North America—and the second biggest ever in the world, measuring 9.2 on the Richter scale—struck Alaska, devastating coastal towns and villages and killing more than 130 people in what was then a relatively sparsely populated region. In a riveting tale about the almost unimaginable brute force of nature, New York Times science journalist Henry Fountain, in his first trade book, re-creates the lives of the villagers and townspeople living in Chenega, Anchorage, and Valdez; describes the sheer beauty of the geology of the region, with its towering peaks and 20-mile-long glaciers; and reveals the impact of the quake on the towns, the buildings, and the lives of the inhabitants. George Plafker, a geologist for the U.S. Geological Survey with years of experience scouring the Alaskan wilderness, is asked to investigate the Prince William Sound region in the aftermath of the quake, to better understand its origins. His work confirmed the then controversial theory of plate tectonics that explained how and why such deadly quakes occur, and how we can plan for the next one.

    Scientists around the world are currently monitoring the sixth extinction, predicted to be the most devastating extinction event since the asteroid impact that wiped out the dinosaurs. This time around, the cataclysm is us. In prose that is at once frank, entertaining, and deeply informed, The New Yorker writer Elizabeth Kolbert tells us why and how human beings have altered life on the planet in a way no species has before. Interweaving research in half a dozen disciplines, descriptions of the fascinating species that have already been lost, and the history of extinction as a concept, Kolbert provides a moving and comprehensive account of the disappearances occurring before our very eyes. She shows that the sixth extinction is likely to be mankind's most lasting legacy, compelling us to rethink the fundamental question of what it means to be human.

    And the moments are part of college basketball lore: the Sweater Game, Villanova Beats Georgetown, and Six Overtimes. But this is the story of the Big East Conference that you haven't heard before--of how the Northeast, once an afterthought, became the epicenter of college basketball.Before the league's founding, East Coast basketball had crowned just three national champions in forty years, and none since 1954. But in the Big East's first ten years, five of its teams played for a national championship. The league didn't merely inherit good teams; it created them. But how did this unlikely group of schools come to dominate college basketball so quickly and completely?Including interviews with more than sixty of the key figures in the conference's history, The Big East charts the league's daring beginnings and its incredible rise. It transports fans inside packed arenas to epic wars fought between transcendent players, and behind locker-room doors where combustible coaches battled even more fiercely for a leg up.Started on a handshake and a prayer, the Big East carved an improbable arc in sports history, an ensemble of Catholic schools banding together to not only improve their own stations but rewrite the geographic boundaries of basketball. As former UConn coach Jim Calhoun eloquently put it, "It was Camelot. Camelot with bad language."

    As a result, East Asian thought is “holistic”—drawn to the perceptual field as a whole and to relations among objects and events within that field. By contrast, Westerners focus on salient objects or people, use attributes to assign them to categories, and apply rules of formal logic to understand their behavior. From feng shui to metaphysics, from comparative linguistics to economic history, a gulf separates the children of Aristotle from the descendants of Confucius. At a moment in history when the need for cross-cultural understanding and collaboration have never been more important, The Geography of Thought offers both a map to that gulf and a blueprint for a bridge that will span it.

    These “psychosurgeons,” as they called themselves, occupied a gray zone between medical research and medical practice, and ended up subjecting untold numbers of people to the types of surgical experiments once limited to chimpanzees.The most important test subject to emerge from this largely untold chapter in American history was a twenty-seven-year-old factory worker named Henry Molaison. In 1953, Henry—who suffered from severe epilepsy—received a radical new version of the lobotomy, one that targeted the most mysterious structures in the brain. The operation failed to eliminate Henry’s seizures, but it did have an unintended effect: Henry left the operating room profoundly amnesic, unable to create new long-term memories. Over the next sixty years, Patient H.M., as Henry was known, became the most studied individual in the history of neuroscience, a human guinea pig who would teach us much of what we know about memory today.Luke Dittrich uses the case of Patient H.M. as a starting point for a kaleidoscopic journey, one that moves from the first recorded brain surgeries in ancient Egypt to the cutting-edge laboratories of MIT. He takes readers inside the old asylums and operating theaters where psychosurgeons conducted their human experiments, and behind the scenes of a bitter custody battle over the ownership of the most important brain in the world. Throughout, Dittrich delves into the enduring mysteries of the mind while exposing troubling stories of just how far we’ve gone in our pursuit of knowledge. It is also, at times, a deeply personal journey. Dittrich’s grandfather was the brilliant, morally complex surgeon who operated on Molaison—and thousands of other patients. The author’s investigation into the dark roots of modern memory science ultimately forces him to confront unsettling secrets in his own family history, and to reveal the tragedy that fueled his grandfather’s relentless experimentation—experimentation that would revolutionize our understanding of ourselves.Patient H.M. combines the best of biography, memoir, and science journalism to create a haunting, endlessly fascinating story, one that reveals the wondrous and devastating things that can happen when hubris, ambition, and human imperfection collide.

    

    It is fundamental to Philosophical Taoism. Specially formatted for the Amazon Kindle, with easy navigation table of contents, and adjustable font size.

    A group of young scientists, under the guidance of Dr. George Church, the most brilliant geneticist of our time, works to make fantasy reality by sequencing the DNA of a frozen woolly mammoth harvested from above the Arctic circle, and splicing elements of that sequence into the DNA of a modern elephant. Will they be able to turn the hybrid cells into a functional embryo and bring the extinct creatures to life in our modern world?Along with Church and his team of Harvard scientists, a world-famous conservationist and a genius Russian scientist plan to turn a tract of the Siberian tundra into Pleistocene Park, populating the permafrost with ancient herbivores as a hedge against an environmental ticking time bomb.