Assuming the reader has little prior knowledge of the subject its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented two-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology: Extended chapter on agriculture including new material on glyphosate resistant plantsNew section on the uses of gene cloning and PCR in archaeologyCoverage of ethical concerns relating to pharming, gene therapy and GM crops Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. View the Gene Cloning and DNA Analysis webpage at www.blackwellpublishing.com/genecloning

    They live everywhere—deep inside caves, 18,000 feet high in the Himalayas, inside computers, in Yellowstone’s hot springs, and in the ears and nostrils of much larger creatures. There are insects that have ears on their knees, eyes on their penises, and tongues under their feet. Most of us think life would be better without bugs. In fact, life would be impossible without them. Most of us know that we would not have honey without honeybees, but without the pinhead-sized chocolate midge, cocoa flowers would not pollinate. No cocoa, no chocolate. The ink that was used to write the Declaration of Independence was derived from galls on oak trees, which are induced by a small wasp. The fruit fly was essential to medical and biological research experiments that resulted in six Nobel prizes. Blowfly larva can clean difficult wounds; flour beetle larva can digest plastic; several species of insects have been essential to the development of antibiotics. Insects turn dead plants and animals into soil. They pollinate flowers, including crops that we depend on. They provide food for other animals, such as birds and bats. They control organisms that are harmful to humans. Life as we know it depends on these small creatures. With ecologist Anne Sverdrup-Thygeson as our capable, entertaining guide into the insect world, we’ll learn that there is more variety among insects than we can even imagine and the more you learn about insects, the more fascinating they become. Buzz, Sting, Bite is an essential introduction to the little creatures that make the world go round.

    After a brief tale of her own introduction to the paranormal, the author shares funny, poignant, and insightful words straight from the spirit people themselves. Together, the living and the dead seek forgiveness, solve family mysteries, find closure, settle scores, and come together for birthdays, anniversaries, and graduations. Quoting directly from her readings and séances, Priscilla reports the spirit perspective on mental illness, suicide, religion, and even the afterlife itself. For those readers interested in developing their own spirit communication skills, the last section of the book offers meditations and exercises used by the author herself, both personally and with her students. "It Will All Make Sense When You’re Dead" is chock-full of simple and entertaining wisdom, showing us how to live for today, with light hearts and kindness.

    and a bike called Reggie. Secondary school teacher Andrew Sykes moves out of the classroom, climbs onto his bicycle and sets off along the route of the EuroVelo 8, from the southern tip of Greece to the Atlantic coast of Portugal.However, this is more than just a cycling tale of border crossings and big hills, as our would-be adventurer perspires his way through a hot and sticky mix of Mediterranean landscapes, life and culture.Join Andrew as he travels Along The Med on a Bike Called Reggie: an inspirational and light-hearted travelogue for cyclists and non-cyclists alike.

    Scott guides readers through the latest advances in stem cell research in clear, accessible language, telling the stories of the researchers who are exploring the potential of stem cells to cure cancer, grow new organs, and repair the immune system. He also leads readers through a discussion of the question at the heart of the explosive ethical debate: How, as a society, do we balance our responsibilities to the unborn and the sick? Stem Cell Now is essential reading for anyone who wants to build an informed opinion on stem cell research.

    Now award-winning writer Carl Zimmer takes us on a fantastic voyage into the secret parasite universe we actually live in but haven't recognized. He reveals not only that parasites are the most successful life-forms on Earth, but that they triggered the development of sex, shape ecosystems, and have driven the engine of evolution. In mapping the parasite universe, Zimmer makes the astonishing observation that most species are parasites, and that almost every animal, including humans, will at one time or another become the home of a parasite. Zimmer shows how highly evolved parasites are and describes the frightening and amazing ingenuity these commando invaders use to devour their hosts from the inside and control their behavior. The sinister Sacculina carcini makes its home in an unlucky crab and proceeds to eat everything but what the crab needs to put food in its mouth, which Sacculina then consumes. When Sacculina finally reproduces, it places its young precisely where the crab would nurture its own progeny, and then has the crab nurture the foster family members. Single-celled Toxoplasma gondi has an even more insidious role, for it can invade the human brain. There it makes men distrustful and less willing to submit to social mores. Women become more outgoing and warm-hearted. Why would a parasite cause these particular personality changes? It seems Toxoplasma wants its host to be less afraid, to be more prone to danger and a violent end -- so that, in the carnage, it will be able to move on to another host. From the steamy jungles of Costa Rica to the fetid parasite heaven of rebel-held southern Sudan, Zimmer tracks the genius of parasitic life and its impact on humanity. We hosts have developed remarkable defenses against the indomitable parasite: our mighty immune system, our culturally enforced habit of keeping clean, and, perhaps most intriguingly, sex. But this is not merely a book about the evil power of parasitism and how we must defend against it. On the contrary, Zimmer concludes that humankind itself is a new kind of parasite, one that preys on the entire Earth. If we are to achieve the sophistication of the parasites on display here in vivid detail, if we are to promote the flourishing of life in all its diversity as they do, we must learn the ways nature lives with itself, the laws of Parasite Rex.

    For millions, the Galápagos Islands represent nature at its most unspoiled, an inviolate place famed for its rare flora and fauna. But soon today’s 30,000 human residents could surpass 50,000. Add invasive species, floods of tourists, and unresolved conflicts between Ecuadorian laws and local concerns, and it’s easy to see why the Galápagos were recently added to UNESCO’s World Heritage in Danger list. Each chapter in this provocative, perceptive book focuses on a specific person or group with a stake in the Galápagos’ natural resources—from tour companies whose activities are often illegal and not always green, to creationist guides who lead tours with no mention of evolution, from fishermen up in arms over lobster quotas, to modern-day pirates who poach endangered marine species. Bassett presents a perspective as readable as it is sensible. Told with wit, passion, and grace, the Galápagos story serves as a miniature model of Earth itself, a perfect example of how an environment can be destroyed-- and what is being done to preserve these islands before it's too late.

    McPhee embarks on a fascinating journey across the basement of the continent -- the land masses forming Illinois, Iowa, Nebraska, and thereabouts -- with a professor and geochronologist acting as a guide.

    Since that time he has answered thousands of questions about birds, both at his store and while walking down the aisles of the supermarket. The questions have ranged from inquiries about individual species ("Are flamingos really real?") to what and when to feed birds ("Should I bring in my feeders for the summer?") to the down-and-dirty specifics of backyard birding ("Why are the birds dropping poop in my pool?"). Answering the questions has been easy; keeping a straight face has been hard.Why Don't Woodpeckers Get Headaches? is the solution for the beginning birder who already has a book that explains the slight variation between Common Ground-Doves and Ruddy Ground-Doves but who is really much more interested in why birds sing at 4:30 A.M. instead of 7:00 A.M., or whether it's okay to feed bread to birds, or how birds rediscover your feeders so quickly when you've just filled them after a long vacation. Or, for that matter, whether flamingos are really real.

    Get paid to travel and write about it.Only no one told Jules that it would mean eating oily seabirds, repeatedly falling off a husky sled, getting stranded on a Mediterranean island, and crash-landing in Iran.The exotic destinations come thick and fast – Hong Kong, Hawaii, Huddersfield – as Jules navigates what it means to be a travel writer in a world with endless surprises up its sleeve.Add in a cast of larger-than-life characters – Elvis, Captain Cook, his own travel-mad Dad – and an eye for the ridiculous, and this journey with Jules is one you won’t want to miss.

    He would return in September. For the next ten weeks my survival would depend on foraging, hunting and fishing on an island I would share with 1,600 brown bears. This is my story of hunger and solitude, salmon fishing and stormy seas, torrential rains and mountain sunsets, giant halibut and deer hunting, campfires and killer whales. Illustrated with nearly fifty photos and a map.

    She knew instantly that her decision to work in general practice was the ‘biggest and worst mistake of her life’. Fortunately, however, this did not deter her from continuing in general practice, and this fascinating memoir (spanning half a century) is testament to her resilience and professionalism, as well as her pragmatic and charismatic personality. She shares real stories about real people in this intriguing book. Some stories are truly heart-breaking and will have you reaching for the tissues (such as the times when she has lost patients, and encountered and supported abused children and rape victims). But it isn’t all serious. There are lots of light-hearted and heart-warming moments too, such as the stories about Jessie-dog – her bodyguard when she made home visits, and the time when she helped a large (and desperately in need) family to get rehoused, and her time as a country GP. She also recalls with honesty and candidness, the prejudice and unimaginable pressure she had to contend with, as a young female GP in the 1960s. As well as a plethora of fascinating stories, experiences and case studies, this book also gives us, as 21st Century readers, a glimpse into the rapid changes in general practice and the NHS in general. Whether you’re in general practice, or you’re a medical professional, or you have a penchant for all kinds of autobiographies/memoirs, you will find this a thought-provoking and captivating book that’s impossible to put down. Take a peek at the ‘Look Inside’ feature now and be prepared to be instantly intrigued.

    But don’t blame yourself if you’ve struggled losing weight. There’s a lot of conflicting information and nonsense out there. Drawing on years of research, Fat Funeral answers critical questions, busts myths, and introduces The Five Golden Weight-Loss Habits—a system of simple, science-based habits that are proven, practical, and powerful enough to completely change your life.