Assuming the reader has little prior knowledge of the subject its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented two-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology: Extended chapter on agriculture including new material on glyphosate resistant plantsNew section on the uses of gene cloning and PCR in archaeologyCoverage of ethical concerns relating to pharming, gene therapy and GM crops Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. View the Gene Cloning and DNA Analysis webpage at www.blackwellpublishing.com/genecloning

    He's the prime suspect in the 2004 murder of Keith Palomares, a 25-year-old armored truck guard. Despite the FBI's active investigation, Brown remains at large living among us without a trace. And yet, a faint pulse of his identity surfaces from time to time, haunting the detectives tasked to find him. In the Kindle Single The Ghost, crime writer Paige Williams chronicles the case and draws a portrait of a killer who is as slippery and elusive as he is enigmatic. Jason Derek Brown was raised by a Mormon father who held a high position in the church despite being a known con man. Jason himself was a devout Mormon for years, and maintained his generosity and Southern California charm even as he slid into a life of excessive materialism fueled by theft. Aside from the murder, he has no history of violence. His case is downright perplexing, and Williams captures it from multiple viewpoints in pitch-perfect prose. --Paul Diamond

    Quickly get to work - or get that job - with the right tools and the best practices.Seriously: Stop wasting your time scouring Google, searching through incorrect, out-of-date, blog posts and get everything you need to be productive in one, well-organized place. The book is complete with both simple and complex examples to get your apps up and running.You'll learn what you need to know to work professionally and build solid, well-tested, optimized apps with ReactJS. This book is your definitive guide or your money back.Buy now at https://www.fullstackreact.com.

     "Before I wrote this book, I was diagnosed as MORBIDLY OBESE by my primary-care physician following a yearly visit. The only problem with this diagnosis was that we never discussed this issue. I knew that I was overweight, but it wasn't until I switched doctors and picked up my medical records that I saw this in writing. I saw myself in front of the mirror every day, so I knew things were bad, but it wasn't until I read those words, MORBIDLY OBESE, that the gravity of the situation sunk in." -LAURA SINCLAIR At 55, Laura Sinclair was grossly overweight; the future looked dim and dangerous. Carrying extra weight was setting her up for the possibility of developing a chronic illness--heart disease, stroke, or worse. She decided to take ownership of her life and pave a new road to optimum health and happiness. -Learn how Laura lost over 40% of her body weight and kept it off. -How she reached a level of fitness and athleticism that she thought not possible in her 50s and beyond.

    When various complications arise during the journey, Nate finds himself focusing closely on the characters around him for a bit of entertainment, but ends up getting more than he bargained for. The focal point of the story is Nate's seat-mate, a young man named Bud, whose extremely odd behavior catches the attention of everyone on the bus.

    A fearless group of young men that made a name for themselves in Chicago’s underworld of gangs and drugs; The Black Corleones—rose to the top ranks of the dope world—all before finishing high school. For them, life was good; money, cars, clothes and women; all at their disposal. But everything isn’t always what it seems. Find out what happens when one member of the crew has ill feelings. Dive into the world of The Black Corleones as they learn the hard way. With money involved; love and loyalty don’t always go hand in hand.

    An uncensored account of his extraordinary true life story and his lifelong struggle to overcome an abusive childhood and build a 'normal' life for himself and his family. Put out with the rubbish at 2 weeks old and into the care system of the London Borough of Tower Hamlets, Paul Connolly was sent to the notorious St Leonard’s home in Essex, where he heard that he was 'Not Normal' almost daily amongst years of mental, physical and sexual abuse. Childhood friendships made and lost. Total Neglect. Starvation. Sending an illiterate, very angry young man out into the world. After years of extreme violence in London during the 1980's and 90's mixing with gangsters and a dark underworld, Paul confronted memories and demons from his past in the most shocking ways - eventually discovering that six from eight of the childhood friends he grew up with in St Leonard's were no longer alive. Great friends and a loving heart transformed Paul’s world and Paul has gone on to help thousands of people as a specialist conditioning coach, sports injury expert, celebrity personal trainer, presenter and best selling author. He works with various charities and is a core participant in the historical child abuse enquiry providing a voice for many of those no longer able to speak up. Above all else, Paul is now most proud of being a loving father to his two sons. Life has been anything but normal and the demons will never go away, but Paul has learned to smile at them as many of the people that predicted the worst for him are now where they told this vulnerable little boy he would end up. This is Paul Connolly’s story and it is Not Normal

    A collection of fascinating facts, tidbits and quotes from the world of science and technology.This is a quick read, which you can easily scan through and find easy to read, short facts about the world around us, as well as some quotes from well known faces in science.Topics in the book include;BiologyChemistryPhysicsHealthSocietyEarth & EnvironmentAnimals & NatureSpaceTechnology & EngineeringQuotes

    Consumer's identities are being stolen, and a person's every step is being tracked and stored. What once might have been dismissed as paranoia is now a hard truth, and privacy is a luxury few can afford or understand.In this explosive yet practical book, Kevin Mitnick illustrates what is happening without your knowledge--and he teaches you "the art of invisibility." Mitnick is the world's most famous--and formerly the Most Wanted--computer hacker. He has hacked into some of the country's most powerful and seemingly impenetrable agencies and companies, and at one point he was on a three-year run from the FBI. Now, though, Mitnick is reformed and is widely regarded as the expert on the subject of computer security. He knows exactly how vulnerabilities can be exploited and just what to do to prevent that from happening. In THE ART OF INVISIBILITY Mitnick provides both online and real life tactics and inexpensive methods to protect you and your family, in easy step-by-step instructions. He even talks about more advanced "elite" techniques, which, if used properly, can maximize your privacy. Invisibility isn't just for superheroes--privacy is a power you deserve and need in this modern age.

    The murders were similar enough that police considered them the result of one person and believed them to be the work of a serial killer. But as quickly as the murders began, the killings seemed to end. Detectives suspected the Spokane River Killer was either dead, incarcerated or had moved out of the area. Six years later, the bodies of murdered women once again began turning up all around the Spokane area. In 1999, Robert Lee Yates was arrested and police hoped they had finally found their killer. In 2000, he plead guilty to killing 11 women from the Spokane area between 1996 and 1998. During the investigation however, Yates was ruled out as a suspect in the 1990 slayings. DNA recovered from underneath the fingernails of the third victim was eventually identified as being from a man. But no match could be found. Their man was still on the loose. In 2009, DNA from a federal inmate was matched to the DNA recovered from underneath the fingernails of the third victim. The inmates fingerprint was also matched to a print recovered from a bottle of vaginal lubricant found in a dumpster along with the second victim’s discarded belongings. Police finally believed they had their man. But did they? The DNA match belonged to a female inmate.

    Only twenty years old and not long qualified as a nurse, she had signed up to serve in the Burma Campaign. She would be based on the Indian border, near the frontline where a fierce battle was raging between Allied forces and the Japanese.As Madge arrived in Chittagong, she wondered how she would adapt to the ever present danger of invasion and to life in a military hospital. She spent long, exhausting hours nursing the badly-injured young soldiers in her care, but found strength in her friendship with the other nurses. And then, one day, she met Captain Basil Lambert . . . Could their fragile, new found romance survive the terrifying final months of war? Heart-warming and poignant, Some Sunny Day by Madge Lambert is a story of courage, sacrifice and the power of true love.

    Today's increasingly fast pace of software development--as programmers make small changes to software one day only to undo them the next--has only heightened the problem; consecutive work on code or single-programmer software is a rare sight these days. Without careful attention to version control, concurrent and collaborative work can create more headaches than it solves. This is where Subversion comes into play.Written by members of the Subversion open source development team, Version Control with Subversion introduces the powerful new versioning tool designed to be the successor to the Concurrent Version System or CVS. CVS users will find the "look and feel" Subversion comfortably familiar, but under the surface it's far more flexible, robust, and usable, and more importantly, it improves on CVS's more notable flaws.The book begins with a general introduction to Subversion, the basic concepts behind version control, and a guided tour of Subversion's capabilities and structure. With thorough attention to detail, the authors cover every aspect of installing and configuring Subversion for managing a programming project, documentation, or any other team-based endeavor. Later chapters cover the more complex topics of branching, repository administration, and other advanced features such as properties, externals, and access control. The book ends with reference material and appendices covering a number of useful topics such as a Subversion complete reference and troubleshooting guide.Version Control with Subversion aims to be useful to readers of widely different backgrounds, from those with no previous experience in version control to experienced sysadmins. If you've never used version control, you'll find everything you need to get started in this book. And if you're a seasoned CVS pro, this book will help you make a painless leap into Subversion.

    Each chapter serves a different purpose. The chapters are For The Scarred, For the Loving, For the Oppressed, and For the Healing. This book teaches you to love yourself and others. It teaches the difficulty of dealing with past traumas and the journey towards healing, despite when it feels as if your scars are set in stone. It's better experienced than described, so sit down with some coffee and allow yourself to feel-- and heal.

    The time you spend context switching between your editor and your consoles eats away at your productivity. Take control of your environment with tmux, a terminal multiplexer that you can tailor to your workflow. With this updated second edition for tmux 2.3, you'll customize, script, and leverage tmux's unique abilities to craft a productive terminal environment that lets you keep your fingers on your keyboard's home row.You have a database console, web server, test runner, and text editor running at the same time, but switching between them and trying to find what you need takes up valuable time and breaks your concentration. By using tmux 2.3, you can improve your productivity and regain your focus. This book will show you how.This second edition includes many features requested by readers, including how to integrate plugins into your workflow, how to integrate tmux with Vim for seamless navigation - oh, and how to use tmux on Windows 10.Use tmux to manage multiple terminal sessions in a single window using only your keyboard. Manage and run programs side by side in panes, and create the perfect development environment with custom scripts so that when you're ready to work, your programs are waiting for you. Manipulate text with tmux's copy and paste buffers, so you can move text around freely between applications. Discover how easy it is to use tmux to collaborate remotely with others, and explore more advanced usage as you manage multiple tmux sessions, add custom scripts into the tmux status line, and integrate tmux with your system.Whether you're an application developer or a system administrator, you'll find many useful tricks and techniques to help you take control of your terminal.

    In SHAPING THE WORLD FROM THE SHADOWS the post-9/11 activities of Delta Force have finally been assembled and put into context, providing a wide-ranging look into the staggering secret history of the world's leading special operations force in their defining hour."Chris Martin has written an astonishing account of special operations activities around the globe. Someone at the Pentagon should check for any missing keys..." - D.B. Grady, co-author of THE COMMAND: DEEP INSIDE THE PRESIDENT'S SECRET ARMY"Chris does an amazing job compiling open source information about this often misunderstood special operations unit, a job that journalists and researchers should have done a long time ago. Delta Force is one of the most secretive organizations in the U.S. military but by aggregating information from dozens of sources Chris has put together a big picture that will give readers an unprecedented behind-the-scenes look at this unit. For sure this is just a small sampling of what is really going on behind the curtain, but until these missions are declassified, this is the best information you are likely to find on the topic." - Jack Murphy, former Ranger and Special Forces Sergeant