Assuming the reader has little prior knowledge of the subject its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented two-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology: Extended chapter on agriculture including new material on glyphosate resistant plantsNew section on the uses of gene cloning and PCR in archaeologyCoverage of ethical concerns relating to pharming, gene therapy and GM crops Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. View the Gene Cloning and DNA Analysis webpage at www.blackwellpublishing.com/genecloning

    Musical Theatre: A History presents a comprehensive history of stage musicals from the earliest accounts of the ancient Greeks and Romans, for whom songs were common elements in staging, to Jacques Offenbach in Paris during the 1840s, to Gilbert and Sullivan in England, to the rise of music halls and vaudeville traditions in America, and eventually to "Broadway's Golden Age" with George M. Cohan, Victor Herbert, Jerome Kern, George and Ira Gershwin, Rodgers and Hart, Oscar Hammerstein, Leonard Bernstein, and Andrew Lloyd Webber. The 21st century has also brought a popular new wave of musicals to the Broadway stage, from The Producers to Spamalot, and Mamma Mia! to The Drowsy Chaperone. Musical Theatre: A History covers it all, from the opening number to the curtain call, offering readers the most comprehensive and up-to-date history of the art form. As informative as it is entertaining, Musical Theatre is richly illustrated with anecdotes of shows and show people. It is cause for celebration for those working in the theatre as well as its legion of devoted fans.

    The turbulent political weather of the twenty-first century generates anxiety and makes it difficult to know how to react. Olivia Laing makes a brilliant, inspiring case for why art matters more than ever, as a force of both resistance and repair. Art, she argues, changes how we see the world. It gives us X-ray vision. It reveals inequalities and offers fertile new ways of living.Funny Weather brings together a career’s worth of Laing’s writing about art and culture, and their role in our political and emotional lives. She profiles Jean-Michel Basquiat and Georgia O’Keeffe, interviews Hilary Mantel and Ali Smith, writes love letters to David Bowie and Wolfgang Tillmans, and explores loneliness and technology, women and alcohol, sex and the body. With characteristic originality and compassion, Funny Weather celebrates art as an antidote to a terrifying political moment.

    Judith Halberstam proposes “low theory” as a mode of thinking and writing that operates at many different levels at once. Low theory is derived from eccentric archives. It runs the risk of not being taken seriously. It entails a willingness to fail and to lose one’s way, to pursue difficult questions about complicity, and to find counterintuitive forms of resistance. Tacking back and forth between high theory and low theory, high culture and low culture, Halberstam looks for the unexpected and subversive in popular culture, avant-garde performance, and queer art. She pays particular attention to animated children’s films, revealing narratives filled with unexpected encounters between the childish, the transformative, and the queer. Failure sometimes offers more creative, cooperative, and surprising ways of being in the world, even as it forces us to face the dark side of life, love, and libido.

    The indie music world is littered with females who have not only withstood the racket of punk’s intolerance, but have twisted our societal notions of femininity in knots.Raha focuses on the United States and England in the 70s and 80s, and illuminates how the seminal women of this time shaped the female rockers of the 90s and today. Groups profiled range from The Runaways, The Slits, and The Plasmatics to L7, Sleater-Kinney, and Le Tigre. The book includes women not often featured in "women in rock" titles, such as Exene Cervenka of X, Eve Libertine and Joy de Vivre of Crass, and Poison Ivy Rorschach of the Cramps. Includes rare interviews and more than forty B&W photos.

    Weeks later, he was accused of groping a campaign rival. In March, he was asked to leave a gala for being too intoxicated; in May fired as the coach of a high school football team. The events were part of a stream of Rob Ford “mishaps,” which include DUIs, accusations of domestic violence, and a trial where the Toronto City Council stripped him of his powers.Through it all, Ford’s former chief of staff, Mark Towhey, stood by his side. Towhey was part of Ford’s inner circle; he’d joined Ford’s mayoral campaign in 2010 and quickly became one of his closest advisors. He responded to media questions regarding Ford’s drug and alcohol additions, his anger management problems, and, of course, the video of Ford smoking crack. In May 2013, Mark Towhey had a confidential conversation with Ford. It was shortly after the video was made public and also followed rumors of Ford's involvement in the murder of Anthony Smith, who stands beside Ford in the video. Thus far, the public only knows two words from that conversation; Towhey told Ford to “get help.” They also know what happened next, Towhey was fired. In Uncontrollable: My Life with Mayor Rob Ford, Towhey gives an insider account of working with Ford, covering for him, managing a man who people see as a joke, who trips over himself in videos; who throws candy at children instead of handing it to them; who rants and raves, and gets belligerent in meetings and at private events.This is a must-read for Canadians voting in the mayoral election, as well as fans of Ford—and his antics—all over the world. It’s an unparalleled tell-all and perhaps what’s most amazing is that Towhey bears no ill will toward the mayor. This is not the account of a man eager to get revenge. It’s simply an up-close look at the mayor—and what goes on behind the scenes.

    They don’t want to be Barbie dolls but, like generations of women before them, are told they must look like them. They’re angry about the media’s treatment of women but hungrily consume the very outlets that belittle them. They mock modern culture’s absurd beauty ideal and make videos exposing Photoshopping tricks, but feel pressured to emulate the same images they criticize by posing with a "skinny arm." They understand that what they see isn’t real but still download apps to airbrush their selfies. Yet these same young women are fierce fighters for the issues they care about. They are ready to fight back against their beauty-sick culture and create a different world for themselves, but they need a way forward.In Beauty Sick, Dr. Renee Engeln, whose TEDx talk on beauty sickness has received more than 250,000 views, reveals the shocking consequences of our obsession with girls’ appearance on their emotional and physical health and their wallets and ambitions, including depression, eating disorders, disruptions in cognitive processing, and lost money and time. Combining scientific studies with the voices of real women of all ages, she makes clear that to truly fulfill their potential, we must break free from cultural forces that feed destructive desires, attitudes, and words—from fat-shaming to denigrating commentary about other women. She provides inspiration and workable solutions to help girls and women overcome negative attitudes and embrace their whole selves, to transform their lives, claim the futures they deserve, and, ultimately, change their world.Duration: 11 hr., 45 min., 39 sec.

    These queer thinkers, writers, and artists are committed to undermining a stunted conception of “equality.” In this powerful book, they challenge mainstream gay and lesbian struggles for inclusion in elitist and inhumane institutions. More than a critique, Against Equality seeks to reinvigorate the queer political imagination with fantastic possibility!

    Attaining feminine perfection meant conforming to a mythical standard, one that would come wrapped in an adorable pink package, if those cunning marketers were to be believed. With wise humor and a savvy eye for curious, absurd, and at times wildly funny period artifacts, Lynn Peril gathers here the memorabilia of the era — from kitschy board games and lunch boxes to outdated advice books and health pamphlets — and reminds us how media messages have long endeavored to shape women's behavior and self-image, with varying degrees of success.Vividly illustrated with photographs of vintage paraphernalia, this entertaining social history revisits the nostalgic past, but only to offer a refreshing message to women who lived through those years as well as those who are coming of age now.

    Throughout the world, she is associated with a peaceful struggle for democracy and human rights. But what is she really like? What drives her to make such enormous personal sacrifices for her country?Jesper Bengtsson presents a portrait of one of today’s most significant political activists. He chronicles her background as the daughter of Burma’s liberation hero Aung San, the years she spent in England and New York, and her return to Burma in the 1980s. First placed under house arrest by the military junta in 1989, she spent fifteen of the subsequent twenty-one years in captivity, separated from her husband and two children.Throughout that period, she remained a unifying figure and activist for Burma’s democracy movement. Awarded the Nobel Peace Prize in 1991, she saw her reputation and her international stature grow the longer she was under house arrest. Upon her release in November 2010, she immediately took up her work with the democracy movement and proved that she remains the most important political force in Burma.Aung San Suu Kyi’s ability to affect people and repressive regimes reflects not only her personal charisma and courage but also her devotion to one of the great issues of our times: What is necessary for democracy to evolve from a deeply authoritarian system?

    Drawing from his own life and business triumphs, Schussler offers would-be entrepreneurs a new way of utilizing creativity to achieve their dreams. Schussler distills his principles for entrepreneurs on a budget, and also reveals the ways in which his lessons-from self-branding to developing strategic partnerships to giving recognition where recognition is due-can work in larger corporations. Just like his famous themed restaurants, Schussler's insights provide entertainment, education, and ample food for thought for all business people aspiring to their next level of success.

    Students lashing out at any speaker brave enough to say something they disagree with. Precious snow flakes demanding “Safe Spaces” to protect them from any idea they haven’t heard from their liberal professors. In this book and the accompanying movie, Dennis Prager, Mark Joseph, and Adam Carolla expose the attack on free speech and free thought. It began in the universities, but—fair warning—it’s coming to your neighborhood and your workplace. “No Safe Spaces is a film every American should see. I could barely move when it was over. Powerful, emotional, and a call to action for anyone worried about the intellectual fascism happening in this country. A brave, timely, and important film.” —MEGYN KELLY, former FOX News anchor and host of Megyn Kelly Today “There is no free speech in America for free thinkers! You can have free speech in America but only if you say what everybody else agrees with. It’s not enough to ‘live and let live’ now. The psycho-elite believe ‘silence is violence’ and you must actively promote what THEY want no matter how vile or reprehensible it is to you. George Orwell lives! They should’ve called Orwell ‘Nostradamus’ because his most frightening prophecies have come to pass, as you will witness in No Safe Spaces!” —MANCOW MULLER, radio phenomenon “An excellent film, the best I’ve seen on the subject of free speech. I especially like Dennis’s line, ‘They have to believe we are evil; otherwise they’d have to debate us.’ Perfect!” —CAL THOMAS, America’s #1 syndicated columnist

    . . Roemer [is] America's most decorated FBI agent."--Chicago TribuneFor forty years Tony Accardo was America's most dangerous criminal. He cut his teeth on the Chicago mob wars of Capone and Elliot Ness. He got his nickname "Joe Batters" for killing two men with a baseball bat. As the bodies piled up, Capone's youngest capo murdered and schemed his way to the top.William Roemer was the first FBI agent to face Tony "The Big Tuna" Accardo. Now, Roemer tells the story that only he could tell: the deals, the hits, the double-crosses, and the power plays that reached from the Windy City to Hollywood and to New York. Drawing on secret wiretaps and inside information, ACCARDO chronicles bloodshed and mayhem for more than six decades--as Roemer duels against the most powerful don of them all. . . ."Roemer brings the reality of organized crime home to us."--Boston Herald"A big, sprawled out account that serves as anecdotal history of organized crime."--Kirkus Reviews

    You may find it for free on the web. Purchase of the Kindle edition includes wireless delivery.

    In her brief yet fruitful life, she was variously author, editor, literary and social critic, journalist, poet, and revolutionary. She was also one of the few female members of the prestigious Transcendentalist movement, whose ranks included Ralph Waldo Emerson, Henry David Thoreau, Elizabeth Palmer Peabody, Nathaniel Hawthorne, and many other prominent New England intellectuals of the day. As co-editor of the transcendentalist journal, The Dial, Fuller was able to give voice to her groundbreaking social critique on woman's place in society, the genesis of the book that was later to become Woman in the Nineteenth Century. Published in 1843, this essay was entitled "The Great Lawsuit: Man versus Men, Woman versus Women."First published in book form in 1845, Woman in the Nineteenth Century was correctly perceived as the controversial document that it was: receiving acclaim and achieving popular success in some quarters (the first printing sold out within a week), at the same time that it inspired vicious attacks from opponents of the embryonic women's movement. In this book, whose style is characterized by the trademark textual diversity of the transcendentalists, Fuller articulates values arising from her passionate belief in justice and equality for all humankind, with a particular focus on women. Although her notion of basic rights certainly includes those of an educational, economic, and legal nature, it is intellectual expansion and changes in the prevailing attitudes towards women (by men and women) that Fuller cherishes far above the superficial manifestations of liberation. A classic of feminist thought that helped bring about the Seneca Falls Women's Convention three years after its publication, Woman in the Nineteenth Century inspired her contemporaries Elizabeth Cady Stanton and Susan B. Anthony to speak of Fuller as possessing "more influence upon the thought of American women than any woman previous to her time."